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Validation Data Gallery

  • <b>Pulldown of spiked Strep-mStayGold, His-SUMO3-mBaoJin, and His-SUMO3-mStayGold2 from HZ293S cell lysate by MG-3233MA.</b> <br>5 µg of each antigen was spiked into lysate derived from 1.2*10^7 cells. For Coomassie and Western Blot analysis 1% of input (IN), 1% of flowthrough (F), and 20% of bound (B) fraction was loaded. His-SUMO3-mBaoJin and His-SUMO3-mStayGold2 were partially cleaved by intrinsic SUMO-proteases and cleaved mBaoJin or mStayGold2 is present in the bound fraction. Western Blots verify pulldown of Strep-mStayGold, His-SUMO3-mBaoJin and His-SUMO3-mStayGold2.

    Pulldown of spiked Strep-mStayGold, His-SUMO3-mBaoJin, and His-SUMO3-mStayGold2 from HZ293S cell lysate by MG-3233MA.
    5 µg of each antigen was spiked into lysate derived from 1.2*10^7 cells. For Coomassie and Western Blot analysis 1% of input (IN), 1% of flowthrough (F), and 20% of bound (B) fraction was loaded. His-SUMO3-mBaoJin and His-SUMO3-mStayGold2 were partially cleaved by intrinsic SUMO-proteases and cleaved mBaoJin or mStayGold2 is present in the bound fraction. Western Blots verify pulldown of Strep-mStayGold, His-SUMO3-mBaoJin and His-SUMO3-mStayGold2.

  • <b>Pulldown of StayGold, mBaoJin, and the fusion protein Aurora kinase B-mStayGold from transfected HEK 293T cells lysate by MG-3233MA.</b> <br>Each IP was performed with lysate derived from 1x10^7 cells. A mock control with lysate from untransfected cells was included to demonstrate the low background binding of the resin. For Coomassie analysis of the SDS-gel 1% of input (IN), 1% of flowthrough (F), and 10% of bound (B) and Mock fraction was loaded.

    Pulldown of StayGold, mBaoJin, and the fusion protein Aurora kinase B-mStayGold from transfected HEK 293T cells lysate by MG-3233MA.
    Each IP was performed with lysate derived from 1x10^7 cells. A mock control with lysate from untransfected cells was included to demonstrate the low background binding of the resin. For Coomassie analysis of the SDS-gel 1% of input (IN), 1% of flowthrough (F), and 10% of bound (B) and Mock fraction was loaded.

ChromoTek mStayGold-Trap Magnetic Agarose

The ChromoTek mStayGold-Trap Magnetic Agarose consists of an anti-mStayGold VHH (Nanobody), which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of mStayGold or mBaoJin tagged proteins from cell extracts of various organisms.
Cat No. MG-3233MA

Reactivity

mStayGold, mBaoJin, mStayGold2, StayGold

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Magnetic Agarose

Synonyms

QC2-6 FIQ, mStayGold, mStayGold2, mBaojin

Formats:  Magnetic Agarose
Size: 

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Product Information

The ChromoTek mStayGold-Trap Magnetic Agarose consists of an anti-mStayGold VHH (Nanobody), which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of mStayGold or mBaoJin tagged proteins from cell extracts of various organisms.

DescriptionReady to use VHH coupled Magnetic Agarose Beads for Immunoprecipitation of mStayGold.

• Fast, reliable and efficient one-step Immunoprecipitation

• Ready-to-use

• Stable in harsh buffer conditions

• Suitable for downstream mass spec analysis

• High Specificity

• Low background

ApplicationsIP, Co-IP
ReactivitymStayGold, mBaoJin, mStayGold2, StayGold
Binding Capacity/
Bead concentration6% Slurry
Resin TypeMagnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
Elution buffer2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
Wash Buffer Compatibility1 M Urea, 2 M NaCl, 2% NP40, 5 mM β-mercaptoethanol, 2% Triton X-100, 5 mM TCEP, 5 mM DTT, 0.1% SDS
TypeVHH
ClassRecombinant - Animal free production
HostAlpaca
Affinity280 nM (mStayGold)
240 nM (mBaoJin)
Compatibility with mass spectrometry/
RRID/
Storage Buffer20% Ethanol
Storage Conditionupon arrival store at +4°C / do not freeze!
ShippingAmbient Temp
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