Pulldown of spiked Strep-mStayGold, His-SUMO3-mStayGold2, and His-SUMO3-mBaoJin from HZ293S cell lysate by MG-3233A.
5 µg of each antigen was spiked into lysate derived from 1.2*10^7 cells. For Coomassie and Western Blot analysis 1% of input (IN), 1% of flowthrough (F), and 20% of bound (B) fraction was loaded. His-SUMO3-mStayGold2 and His-SUMO3-mBaoJin were partially cleaved by intrinsic SUMO-proteases and cleaved mStayGold2 or mBaoJin is present in the bound fraction. Western Blots verify pulldown of Strep-mStayGold, His-SUMO3-mStayGold2 and His-SUMO3-mBaoJin.

Pulldown of StayGold, mBaoJin, and the fusion protein Aurora kinase B-mStayGold from transfected HEK 293T cells lysate by MG-3233A.
Each IP was performed with lysate derived from 1x10^7 cells. A mock control with lysate from untransfected cells was included to demonstrate the low background binding of the resin. For Coomassie analysis of the SDS-gel 1% of input (IN), 1% of flowthrough (F), and 10% of bound (B) and Mock fraction was loaded.