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Validation Data Gallery

  • <b>Pulldown of spiked Strep-mStayGold, His-SUMO3-mStayGold2, and His-SUMO3-mBaoJin from HZ293S cell lysate by MG-3233A.</b> <br>5 µg of each antigen was spiked into lysate derived from 1.2*10^7 cells. For Coomassie and Western Blot analysis 1% of input (IN), 1% of flowthrough (F), and 20% of bound (B) fraction was loaded. His-SUMO3-mStayGold2 and His-SUMO3-mBaoJin were partially cleaved by intrinsic SUMO-proteases and cleaved mStayGold2 or mBaoJin is present in the bound fraction. Western Blots verify pulldown of Strep-mStayGold, His-SUMO3-mStayGold2 and His-SUMO3-mBaoJin.

    Pulldown of spiked Strep-mStayGold, His-SUMO3-mStayGold2, and His-SUMO3-mBaoJin from HZ293S cell lysate by MG-3233A.
    5 µg of each antigen was spiked into lysate derived from 1.2*10^7 cells. For Coomassie and Western Blot analysis 1% of input (IN), 1% of flowthrough (F), and 20% of bound (B) fraction was loaded. His-SUMO3-mStayGold2 and His-SUMO3-mBaoJin were partially cleaved by intrinsic SUMO-proteases and cleaved mStayGold2 or mBaoJin is present in the bound fraction. Western Blots verify pulldown of Strep-mStayGold, His-SUMO3-mStayGold2 and His-SUMO3-mBaoJin.

  • <b>Pulldown of StayGold, mBaoJin, and the fusion protein Aurora kinase B-mStayGold from transfected HEK 293T cells lysate by MG-3233A.</b> <br>Each IP was performed with lysate derived from 1x10^7 cells. A mock control with lysate from untransfected cells was included to demonstrate the low background binding of the resin. For Coomassie analysis of the SDS-gel 1% of input (IN), 1% of flowthrough (F), and 10% of bound (B) and Mock fraction was loaded.

    Pulldown of StayGold, mBaoJin, and the fusion protein Aurora kinase B-mStayGold from transfected HEK 293T cells lysate by MG-3233A.
    Each IP was performed with lysate derived from 1x10^7 cells. A mock control with lysate from untransfected cells was included to demonstrate the low background binding of the resin. For Coomassie analysis of the SDS-gel 1% of input (IN), 1% of flowthrough (F), and 10% of bound (B) and Mock fraction was loaded.

ChromoTek mStayGold-Trap Agarose, Kit for Immunoprecipitation

The ChromoTek mStayGold-Trap Agarose, Kit for Immunoprecipitation consists of an anti-mStayGold VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mStayGold or mBaoJin tagged proteins from cell extracts of various organisms.
Cat No. MG-3233AK

Reactivity

mStayGold, mBaoJin, mStayGold2, StayGold

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Synonyms

mStayGold, mBaoJin, mStayGold2, StayGold

Formats:  Agarose kit
Size: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Product Information

The ChromoTek mStayGold-Trap Agarose, Kit for Immunoprecipitation consists of an anti-mStayGold VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mStayGold or mBaoJin tagged proteins from cell extracts of various organisms.

DescriptionReady to use VHH coupled Agarose Beads for Immunoprecipitation of mStayGold.

• Fast, reliable and efficient one-step Immunoprecipitation

• Ready-to-use

• Stable in harsh buffer conditions

• Suitable for downstream mass spec analysis

• High Specificity

• Low background

ApplicationsIP, Co-IP
ReactivitymStayGold, mBaoJin, mStayGold2, StayGold
Binding Capacity/
Bead concentration50% Slurry
Resin TypeAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
Wash Buffer Compatibility1 M Urea, 1 M NaCl, 2% NP40, 5 mM β-mercaptoethanol, 2% Triton X-100, 5 mM TCEP, 5 mM DTT, 0.1% SDS
TypeVHH
ClassRecombinant - Animal free production
HostAlpaca
Affinity280 nM (mStayGold)
240 nM (mBaoJin)
Compatibility with mass spectrometry/
RRID/
Storage Buffer20% Ethanol
Storage Condition+4°C / do not freeze!
ShippingAmbient Temp

Kit components

Component Description
mStayGold-Trap Agarose20 reactions (500 µl)
Lysis bufferOptimized for cytoplasmic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
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