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Validation Data Gallery

  • SPOT tag related products

    SPOT tag related products

  • Left: HeLa cells transiently expressing pSpot Spot-Tag?-Actin (ev-31) were immunostained with Spot-Label? Alexa Fluor? 647 (magenta, ebAF647, 1:800) and DAPI (blue). Right: Control staining, no background noise; HeLa cells without pSpot Spot-Tag?-Actin expression were immunostained with Spot-Label? Alexa Fluor? 647 (magenta, ebAF647, 1:800) and DAPI (blue). Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective.

    Left: HeLa cells transiently expressing pSpot Spot-Tag?-Actin (ev-31) were immunostained with Spot-Label? Alexa Fluor? 647 (magenta, ebAF647, 1:800) and DAPI (blue). Right: Control staining, no background noise; HeLa cells without pSpot Spot-Tag?-Actin expression were immunostained with Spot-Label? Alexa Fluor? 647 (magenta, ebAF647, 1:800) and DAPI (blue). Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective.

ChromoTek Spot-Label® Alexa Fluor® 647

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.
Cat No. ebAF647

Specificity

Spot-Tag®

Applications

IF

Conjugate

Alexa Fluor® 647

Host

Alpaca

Synonyms

PDRVRAVSHWSS, Spot, Spot tag, Spot-Label, SPOT-tag

Size/Concentration: 

-/ -

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Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins.

DescriptionImmunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

• Capture and detection tag without compromises in applications

• Higher image resolution

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Strong avidity effect from bivalent form of Spot-VHH

• Fulfills highest requirements on antibody validation: structure and function characterized

ApplicationsIF
HostAlpaca
Specificity/TargetSpot-Tag® (PDRVRAVSHWSS)
ConjugateAlexa Fluor® 647
Physical StateLiquid
Suggested DilutionIF: 1:800
TypeNanobody; Primary antibody
ClassRecombinant
ClonalityMonoclonal
Clone CTK0216
Calculated MW30.3 kDa
RRIDAB_2889376
ImmunogenBeta-catenin, engineered epitope
FormatRecombinant alpaca single domain antibody, bivalent
Affinity (KD)Dissociation constant KD of 6 nM
DOL1 fluorophore per bivalent Nanobody, site-directed conjugation
Spot-Tag® OriginEngineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
Cross ReactivityThe Spot-Nanobody shows minimal cross reactivity with beta-catenin.
Excitation/ EmissionExcitation max: 650 nm, Emission max: 665 nm
Storage BufferPBS, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
Size10 μL; 50 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use