- Featured Product
- KD/KO Validated
CoraLite®594-conjugated PARK2/Parkin Polyclonal antibody
PARK2/Parkin Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-14060
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PARK2 Ready-To-Use IHC Kit. PARK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14060-1-AP
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL594-14060 targets PARK2/Parkin in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PARK2/Parkin fusion protein Ag5092 相同性解析による交差性が予測される生物種 |
| Full Name | Parkinson disease (autosomal recessive, juvenile) 2, parkin |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 42-52 kDa |
| GenBank accession number | BC022014 |
| Gene symbol | PARK2 |
| Gene ID (NCBI) | 5071 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a variety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 PARK2/Parkin antibody CL594-14060 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
