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Validation Data Gallery

  • Immunoprecipitation of GFP-Myc fusion protein from HEK293T cells using Myc-Trap® 2.0 Magnetic Agarose (yt2ma). IP was done using un-transfected (mock) and transfected (GFP-Myc) cells. I: Input, F: Flow-through, B: Bound.

    Immunoprecipitation of GFP-Myc fusion protein from HEK293T cells using Myc-Trap® 2.0 Magnetic Agarose (yt2ma). IP was done using un-transfected (mock) and transfected (GFP-Myc) cells. I: Input, F: Flow-through, B: Bound.

  • Comparison of pulldown efficacy between the Myc-Trap® 2.0 Magnetic Agarose (yt2ma) (left) and the original Myc-Trap Magnetic Agarose (right). Both products were used to immunoprecipitate GFP-myc fusion proteins from untransfected (mock) and transfected (GFP-myc) HEK293T cells. The Myc-Trap 2.0 has a higher binding capacity and is able to pull down more GFP-Myc protein than the Myc-Trap. Pulldowns with the Myc-Trap 2.0 Magnetic Agarose also show significantly reduced background.

    Comparison of pulldown efficacy between the Myc-Trap® 2.0 Magnetic Agarose (yt2ma) (left) and the original Myc-Trap Magnetic Agarose (right). Both products were used to immunoprecipitate GFP-myc fusion proteins from untransfected (mock) and transfected (GFP-myc) HEK293T cells. The Myc-Trap 2.0 has a higher binding capacity and is able to pull down more GFP-Myc protein than the Myc-Trap. Pulldowns with the Myc-Trap 2.0 Magnetic Agarose also show significantly reduced background.

ChromoTek Myc-Trap® 2.0 Magnetic Agarose

The ChromoTek Myc-Trap® 2.0 Magnetic Agarose consists of an anti-Myc NANOBODY®/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of Myc-fusion proteins from cell extracts of various organisms.
Cat No. yt2ma

Specificity

Myc-tag

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Synonyms

Myc tag, Myc, Myc-Trap, EQKLISEEDL

Formats:  Magnetic Agarose
Size: 

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Product Information

The ChromoTek Myc-Trap® 2.0 Magnetic Agarose consists of an anti-Myc NANOBODY®/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of Myc-fusion proteins from cell extracts of various organisms.

DescriptionImmunoprecipitation of myc-tagged proteins and their interacting factors with anti-Myc tag Nanobody conjugated to magnetic agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, yeast, etc.

ApplicationsIP, Co-IP
Specificity/TargetBinds specifically to the Myc-tag (sequence EQKLISEEDL) at the N-terminus,
C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Binding Capacity20 μg of recombinant myc-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
Elution buffer2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5, 0.1 mg/ml ChromoTek 2x Myc-peptide (2yp) in PBS pH 7.4
Wash Buffer Compatibility2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.1% SDS, 1 M Urea
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity (KD)770 nM
Compatibility with mass spectrometryThe Myc-Trap 2.0 is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3717367
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!
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