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Validation Data Gallery

  • Immunoprecipitation of GFP-Myc fusion protein from HEK293T cells using Myc-Trap® 2.0 Magnetic Agarose. IP was done using both untransfected (mock) and transfected (GFP-myc) cells. I: Input, F: Flow-through, B: Bound.

    Immunoprecipitation of GFP-Myc fusion protein from HEK293T cells using Myc-Trap® 2.0 Magnetic Agarose. IP was done using both untransfected (mock) and transfected (GFP-myc) cells. I: Input, F: Flow-through, B: Bound.

  • Comparison of pulldown efficacy between the Myc-Trap® 2.0 Magnetic Agarose (left) and the original Myc-Trap Magnetic Agarose (right). Both products were used to immunoprecipitate GFP-myc fusion proteins from untransfected (mock) and transfected (GFP-myc) HEK293T cells. The Myc-Trap 2.0 has a higher binding capacity and is able to pull down more GFP-Myc protein than the Myc-Trap. Pulldowns with the Myc-Trap 2.0 Magnetic Agarose also show significantly reduced background.

    Comparison of pulldown efficacy between the Myc-Trap® 2.0 Magnetic Agarose (left) and the original Myc-Trap Magnetic Agarose (right). Both products were used to immunoprecipitate GFP-myc fusion proteins from untransfected (mock) and transfected (GFP-myc) HEK293T cells. The Myc-Trap 2.0 has a higher binding capacity and is able to pull down more GFP-Myc protein than the Myc-Trap. Pulldowns with the Myc-Trap 2.0 Magnetic Agarose also show significantly reduced background.

ChromoTek Myc-Trap® 2.0 Magnetic Agarose, Kit for Immunoprecipitation

The ChromoTek Myc-Trap® 2.0 Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-Myc VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of Myc-fusion proteins from cell extracts of various organisms.
Cat No. yt2mak
Publications(1)

Specificity

Myc-tag

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Synonyms

Myc tag, Myc, Myc-Trap, EQKLISEEDL

Formats:  Magnetic Agarose kit
Size: 

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Product Information

The ChromoTek Myc-Trap® 2.0 Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-Myc VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of Myc-fusion proteins from cell extracts of various organisms.

DescriptionThe Myc-Trap 2.0 Magnetic Agarose, Kit for Immunoprecipitation contains Myc-Trap 2.0 Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of myc-tagged proteins.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, yeast, etc.

ApplicationsIP, Co-IP
Specificity/TargetBinds specifically to the Myc-tag (sequence EQKLISEEDL) at the N-terminus,
C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Binding Capacity20 μg of recombinant myc-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
Elution buffer2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5, 0.1 mg/ml ChromoTek 2x Myc-peptide (2yp) in PBS pH 7.4
Wash Buffer Compatibility2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.1% SDS, 1 M Urea
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity (KD)770 nM
Compatibility with mass spectrometryThe Myc-Trap 2.0 is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3717367
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

Component Description
Myc-Trap 2.0 Magnetic Agarose20 reactions (500 µl)
Lysis bufferOptimized for cytoplasmic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
CoIP

Oncogene

Human RNase H2 upregulation counteracts oncogene- and chemotherapy-induced replication stress

Authors - Rosanna J Wilkins
View Article
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