Validation Data Gallery
Product Information
The ChromoTek Myc-Trap® Agarose, Kit for Immunoprecipitation consists of an anti-Myc NANOBODY®/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of Myc-fusion proteins from cell extracts of various organisms.
| Description | The Myc-Trap 2.0 Agarose Kit contains Myc-Trap 2.0 Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of myc-tagged proteins. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to the Myc-tag (sequence EQKLISEEDL) at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound. |
| Binding Capacity | 25 μg of recombinant myc-tagged protein (~30 kDa) per 25 μL bead slurry |
| Conjugate | Agarose beads; ~90 um (cross-linked 4% agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5, 0.1 mg/ml ChromoTek 2x Myc-peptide (2yp) in PBS pH 7.4 |
| Wash Buffer Compatibility | 1M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.2% SDS, 2 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | 770 nM |
| Compatibility with mass spectrometry | The Myc-Trap 2.0 is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3717366 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze! |
Kit components
| Component | Description |
|---|---|
| Myc-Trap 2.0 Agarose | 20 reactions (500 µl) |
| Lysis buffer | Optimized for cytoplasmic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |