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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated BOULE Polyclonal antibody

BOULE Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13720

Synonyms

bol, boule like (Drosophila), BOLL, BOULE, Protein boule like

Validation Data Gallery

Applications:
  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (cold ethanol) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:100, CoraLite®555 TNP1 antibody (CL555-17178, orange), STAR antibody (67130-1-Ig, Clone: 1G6E6, Magenta).

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200.

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200.

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200, CoraLite®594 PRM1 antibody (CL594-15697, red).

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200, CoraLite®594 PRM1 antibody (CL594-15697, red).

Tested Applications

Positive IF detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 13720-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-13720 targets BOULE in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BOULE fusion protein Ag4662 相同性解析による交差性が予測される生物種
Full Name bol, boule-like (Drosophila)
Calculated molecular weight 283 aa, 31 kDa
GenBank accession numberBC033674
Gene symbol BOLL
Gene ID (NCBI) 66037
RRIDAB_2919076
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 BOULE antibody CL488-13720Download protocol
Standard Protocols
Click here to view our Standard Protocols