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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated DAZL Polyclonal antibody (CL594-12633)

    IF Staining of mouse testis using CL594-12633

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 DAZL antibody (CL594-12633) at dilution of 1:400, CoraLite®647 TNP1 antibody (CL647-17178, Magenta), CoraLite®488 BOULE antibody (CL488-13720, green).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated DAZL Polyclonal antibody (CL594-12633)

    IF Staining of mouse testis using CL594-12633

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 DAZL antibody (CL594-12633) at dilution of 1:400, CoraLite®647 TNP1 antibody (CL647-17178, Magenta), CoraLite®488 BOULE antibody (CL488-13720, green).

CoraLite®594-conjugated DAZL Polyclonal antibody

DAZL Polyclonal Antibody for IF-P
Cat No. CL594-12633

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

DAZ homolog, DAZ like autosomal, DAZH, DAZL, DAZL1, DAZLA, deleted in azoospermia like, Deleted in azoospermia like 1, SPGY like autosomal, SPGYLA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-12633 targets DAZL in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3326

Product name: Recombinant human DAZL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-295 aa of BC027595

Sequence: MSTANPETPNSTISREASTQSSSAATSQGYILPEGKIMPNTVFVGGIDVRMDETEIRSFFARYGSVKEVKIITDRTGVSKGYGFVSFFNDVDVQKIVESQINFHGKKLKLGPAIRKQNLCAYHVQPRPLVFNHPPPPQFQNVWTNPNTETYMQPTTTMNPITQYVQAYPTYPNSPVQVITGYQLPVYNYQMPPQWPVGEQRSYVVPPAYSAVNYHCNEVDPGAEVVPNECSVHEATPPSGNGPQKKSVDRSIQTVVSCLFNPENRLRNSVVTQDDYFKDKRVHHFRRSRAMLKSV

相同性解析による交差性が予測される生物種
Full Name deleted in azoospermia-like
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC027595
Gene Symbol DAZL
Gene ID (NCBI) 1618
RRIDAB_2919786
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92904
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 DAZL antibody CL594-12633Download protocol
Standard Protocols
Click here to view our Standard Protocols