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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated PRM1 Polyclonal antibody (CL594-15697)

    IF Staining of mouse testis using CL594-15697

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 PRM1 antibody (CL594-15697) at dilution of 1:200.

CoraLite®594-conjugated PRM1 Polyclonal antibody

PRM1 Polyclonal Antibody for IF-P
Cat No. CL594-15697

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

P1, Cysteine-rich protamine, CT94.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-15697 targets PRM1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8323

Product name: Recombinant human PRM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-51 aa of BC003673

Sequence: MARYRCCRSQSRSRYYRQRQRSRRRRRRSCQTRRRAMRCCRPRYRPRCRR

相同性解析による交差性が予測される生物種
Full Name PRM1
Calculated molecular weight 51 aa, 6 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC003673
Gene Symbol PRM1
Gene ID (NCBI) 5619
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodAntigen Affinity Purified
UNIPROT IDP04553
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRM1 is a 51 amino acid protein. PRM1 substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823 )

Protocols

Product Specific Protocols
IF protocol for CL594 PRM1 antibody CL594-15697Download protocol
Standard Protocols
Click here to view our Standard Protocols