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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UNC13A Monoclonal antibody

UNC13A Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C3A9

Cat no : 68483-1-Ig

Synonyms

KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A

Validation Data Gallery

Applications:
  • WB analysis using 68483-1-Ig

    WB analysis using 68483-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68483-1-Ig (UNC13A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68483-1-Ig

    IHC staining of mouse brain using 68483-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68483-1-Ig (UNC13A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68483-1-Ig

    IHC staining of mouse brain using 68483-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68483-1-Ig (UNC13A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 68483-1-Ig

    IF Staining of SH-SY5Y using 68483-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using UNC13A antibody (68483-1-Ig, Clone: 3C3A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68483-1-Ig targets UNC13A in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name unc-13 homolog A (C. elegans)
Calculated molecular weight 193 kDa
Observed molecular weight193-210 kDa
GenBank accession numberNM_001080421
Gene symbol UNC13A
Gene ID (NCBI) 23025
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

Protocols

Product Specific Protocols
WB protocol for UNC13A antibody 68483-1-IgDownload protocol
IHC protocol for UNC13A antibody 68483-1-IgDownload protocol
IF protocol for UNC13A antibody 68483-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols