CoraLite® Plus 488-conjugated UNC13A Monoclonal antibody
UNC13A Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig, rabbit
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C3A9
Cat no : CL488-68483
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-68483 targets UNC13A in IF applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | Human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | unc-13 homolog A (C. elegans) |
| Calculated molecular weight | 193 kDa |
| Observed molecular weight | 193-210 kDa |
| GenBank accession number | NM_001080421 |
| Gene symbol | UNC13A |
| Gene ID (NCBI) | 23025 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 UNC13A antibody CL488-68483 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
