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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated UNC13A Monoclonal antibody

UNC13A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C3A9

Cat no : CL594-68483

Synonyms

KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A

Validation Data Gallery

Applications:
  • IF Staining of SH-SY5Y using CL594-68483

    IF Staining of SH-SY5Y using CL594-68483

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®594 UNC13A antibody (CL594-68483, Clone: 3C3A9 ) at dilution of 1:400.

Tested Applications

Positive IF detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 68483-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL594-68483 targets UNC13A in IF applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name unc-13 homolog A (C. elegans)
Calculated molecular weight 193 kDa
Observed molecular weight193-210 kDa
GenBank accession numberNM_001080421
Gene symbol UNC13A
Gene ID (NCBI) 23025
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

Protocols

Product Specific Protocols
IF protocol for CL594 UNC13A antibody CL594-68483Download protocol
Standard Protocols
Click here to view our Standard Protocols