• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-19851

Synonyms

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, STING, TMEM173, transmembrane protein 173

Validation Data Gallery

Applications:
  • IF Staining of HT-29 using CL594-19851

    IF Staining of HT-29 using CL594-19851

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-19851

    IF Staining of human tonsillitis using CL594-19851

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-19851

    IF Staining of human tonsillitis using CL594-19851

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

  • IF Staining of HepG2 using CL594-19851

    IF Staining of HepG2 using CL594-19851

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-19851

    FC experiment of HepG2 using CL594-19851

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of THP-1 using CL594-19851

    FC experiment of THP-1 using CL594-19851

    1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of NIH/3T3 using CL594-19851

    FC experiment of NIH/3T3 using CL594-19851

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHT-29 cells, human tonsillitis tissue, HepG2 cells
Positive FC detected inTHP-1 cells, HepG2 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19851-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

CL594-19851 targets TMEM173/STING in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM173/STING fusion protein Ag13921 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 35-40 kDa, 80 kDa
GenBank accession numberBC047779
Gene symbol TMEM173
Gene ID (NCBI) 340061
RRIDAB_2919854
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

Protocols

Product Specific Protocols
IF protocol for CL594 TMEM173/STING antibody CL594-19851Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Neuroinflammation

The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage

Authors - Xingfei Zhu
View Article