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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

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  • 1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed with 1X Transcription Factor Fix/Perm buffer (PF00011-A) and permeabilized with 1X Flow Cytometry Perm Buffer. Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).<br>

    1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed with 1X Transcription Factor Fix/Perm buffer (PF00011-A) and permeabilized with 1X Flow Cytometry Perm Buffer. Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).

Flow Cytometry Perm Buffer (10X)

Cat No. PF00011-C

Concentration:

10x

Applications:

FC

Size: 

-/ -

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Product Information

Flow Cytometry Perm Buffer (10X) Provided as a concentrate which, when diluted with distilled water to a 1X solution, provide best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps.

Storage

4°C

Expiration Date

12 months after receiving.

Cited in Article as

pf00011-c, Flow Cytometry Perm Buffer (10X), Proteintech, IL, USA

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