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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNRHR Polyclonal antibody

GNRHR Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22462-1-AP

Synonyms

GnRH R, GnRH receptor, GNRHR, GNRHR1, GRHR, LHRHR, LRHR

Validation Data Gallery

Applications:
  • WB analysis of mouse ovary using 22462-1-AP

    WB analysis of mouse ovary using 22462-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22462-1-AP (GNRHR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 22462-1-AP

    WB analysis of mouse testis using 22462-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22462-1-AP (GNRHR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22462-1-AP

    IHC staining of human testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 22462-1-AP

    IHC staining of human testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human pituitary adenoma using 22462-1-AP

    IHC staining of human pituitary adenoma using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary adenoma using 22462-1-AP

    IHC staining of human pituitary adenoma using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 22462-1-AP

    IHC staining of rat testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of rat testis using 22462-1-AP

    IHC staining of rat testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse testis using 22462-1-AP

    IHC staining of mouse testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 22462-1-AP

    IHC staining of mouse testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22462-1-AP (GNRHR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 22462-1-AP

    IHC staining of human testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 22462-1-AP

    IHC staining of human testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse ovary tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, rat testis tissue, mouse testis tissue, human pituitary adenoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

22462-1-AP targets GNRHR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNRHR fusion protein Ag18005 相同性解析による交差性が予測される生物種
Full Name GnRH receptor
Calculated molecular weight 328 aa, 38 kDa
Observed molecular weight 60 kDa, 50 kDa
GenBank accession numberBC113546
Gene symbol GNRHR
Gene ID (NCBI) 2798
RRIDAB_2879109
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The antibody only recognizes the isoform1 of GNRHR. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.

Protocols

Product Specific Protocols
WB protocol for GNRHR antibody 22462-1-APDownload protocol
IHC protocol for GNRHR antibody 22462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols