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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNRHR2 Polyclonal antibody

GNRHR2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20728-1-AP

Synonyms

GnRH II R, GNRHR2

Validation Data Gallery

Applications:
  • WB analysis of human brain using 20728-1-AP

    WB analysis of human brain using 20728-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20728-1-AP (GNRHR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human stomach using 20728-1-AP

    WB analysis of human stomach using 20728-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 20728-1-AP (GNRHR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 20728-1-AP

    WB analysis of human placenta using 20728-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20728-1-AP (GNRHR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20728-1-AP

    IHC staining of human kidney using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

  • IHC staining of human liver cancer using 20728-1-AP

    IHC staining of human liver cancer using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20728-1-AP (GNRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 20728-1-AP

    IHC staining of human liver cancer using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20728-1-AP (GNRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 20728-1-AP

    IHC staining of human brain using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

  • IHC staining of human brain using 20728-1-AP

    IHC staining of human brain using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

  • IHC staining of human kidney using 20728-1-AP

    IHC staining of human kidney using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

  • IF Staining of HEK-293 using 20728-1-AP

    IF Staining of HEK-293 using 20728-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20728-1-AP (GNRHR2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inhuman brain tissue, human placenta tissue, human stomach tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20728-1-AP targets GNRHR2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name gonadotropin-releasing hormone (type 2) receptor 2
Calculated molecular weight 33 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberNR_002328
Gene symbol GNRHR2
Gene ID (NCBI) 114814
RRIDAB_2878728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GNRHR2 antibody 20728-1-APDownload protocol
IHC protocol for GNRHR2 antibody 20728-1-APDownload protocol
IF protocol for GNRHR2 antibody 20728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols