Flow Cytometry Applications
Learn about the various applications the flow cytometry is used for.
リンク
- Introduction to flow cytometry
- Flow Cytometry Applications
- Flow Cytometry Fluorophores and Dyes
- Flow Cytometry Multicolor Panel Design
- Flow Cytometry Sample Preparation
- Protocol for studying extracellular and intracellular proteins
- Experimental protocol to study cell viability and apoptosis
- Popular antibodies for flow cytometry
Flow cytometry applications
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Analysis of plasma membrane receptor expression
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Analysis of cell viability, apoptosis, and necrosis
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Cell phenotyping
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Analysis of cell cycle
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Cell sorting
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Analysis of intracellular protein expression
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Biomarker detection
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Cell counting
- Quantification and isolation of Circulation Tumor Cells (CTCs)
Table of cell-line-specific markers to identify or isolate CTCs by Flow cytometry:
Cell line |
Cancer Type |
Flow Cytometry marker |
|
A549 |
NSCLC (Adenocarcinoma) |
|
|
NCI – H226 |
NSCLC (SCC) |
|
|
NCI – H460 |
NSCLC (LCC) |
PD-L1, CD318, EpCAM, Cytokeratin, HPRT |
|
NCI – H69 |
SCLC |
|
|
MCF7 |
BC Luminal A (ER+/PR+) |
CD166, Cytokeratin, CK18, CK19, E-Cadherin, CD24, EpCAM |
|
T47D |
BC Luminal A (ER+/PR+) |
Cytokeratin, E-Cadherin, CK19, CD24, EpCAM, |
|
SKBR3 |
BC ER-/PR- HER2+ |
|
|
AU565 |
BC ER-/PR- HER2+ |
|
|
MDA-MB-231 |
TNBC/Claudin Low |
|
|
MDA-MB-468 |
TNBC |
|
|
4T1 |
Mouse TNBC |
|
|
Hep3B |
HCC |
|
|
HepG2 |
Hepatoblastoma |
|
|
HuH6 |
Hepatoblastoma |
|
|
PC3 |
Prostate |
|
|
DU145 |
Prostate |
|
|
LNCaP |
Prostate |
|
|
ES-2 |
Ovarian |
|
|
PEO-1 |
Ovarian |
|
|
OVCAR-3 |
Ovarian |
|
|
Colorectal Primary CTCs |
Colorectal |
|
|
HCT116 |
Colorectal |
|
|
HT-29 |
Colorectal |
EpCAM, CK20, CD26, CD44, E-Cadherin |
|
T84 |
Colorectal |
|
|
SKMel24 |
Melanoma |
|
|
SkMel28 |
Melanoma |
|
|
WM2664 |
Melanoma |
|
|
AML t(8:21) (patients) |
Leukemia |
|
|
AML t(15:17) (patients) |
Leukemia |
|
|
AML inv (16) (patients) |
Leukemia |
|
|
AML cell lines (U937, KG1a, MV4-11) |
AML |
|
|
(OSU-CLL) |
CLL Chronic Lymphocytic Leukemia – immortalised patient sample |
|
|
Non Hodgkin’s Lymphoma |
Lymphoma |
|
|
Hodgkin’s Lymphoma |
Lymphoma |
|
|
Burkitt’s Lymphoma |
Lymphoma |
Abbreviations:
NSCLC – non small cell lung carcinoma
SCC – squamous cell carcinoma
LCC – large cell carcinoma
SCLC – small cell lung carcinoma
BC – breast cancer
TNBC – triple negative breast cancer
HCC – hepatocellular carcinoma
AML – acute myeloid leukemia
CLL – chronic lymphocytic leukemia
