Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using a-SMA specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human smooth muscle actin specific (67735-1-Ig, Clone:1E9A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
