Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IF | See 26 publications below |
Product Information
68065-1-Ig targets EEA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28814 Product name: Recombinant human EEA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1151-1350 aa of NM_003566 Sequence: KLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWA 相同性解析による交差性が予測される生物種 |
| Full Name | early endosome antigen 1 |
| Calculated molecular weight | 162 kDa |
| Observed molecular weight | 162 kDa |
| GenBank accession number | NM_003566 |
| Gene Symbol | EEA1 |
| Gene ID (NCBI) | 8411 |
| RRID | AB_2918806 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15075 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EEA1 antibody 68065-1-Ig | Download protocol |
| IHC protocol for EEA1 antibody 68065-1-Ig | Download protocol |
| WB protocol for EEA1 antibody 68065-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Arachidonic acid triggers spermidine synthase secretion from primary tumor to induce skeletal muscle weakness upon irradiation | ||
Immunity Modulation of the mevalonate pathway by TCR engagement regulates T follicular helper cell generation in homeostasis and autoimmunity. | ||
Cell Mol Immunol Macrophage CCRL2 promotes NLRP3 inflammasome activation to exacerbate atherosclerosis | ||
Autophagy S-palmitoylation coordinates the trafficking of ATG9A to mediate autophagy initiation | ||
Nat Commun A tissue-intrinsic mechanism sensitizes HIV-1 particles for TLR-triggered innate immune responses. | ||
Nat Chem Biol Oncometabolite 5-IP(7) inhibits inositol 5-phosphatase to license E-cadherin endocytosis. |