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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TLR4 Monoclonal antibody (66350-1-Ig)

    WB analysis using 66350-1-Ig (same clone as 66350-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using TLR4 Monoclonal antibody (66350-1-Ig)

    WB analysis of RAW 264.7 using 66350-1-Ig (same clone as 66350-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using TLR4 Monoclonal antibody (66350-1-Ig)

    WB analysis of pig liver using 66350-1-Ig (same clone as 66350-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using TLR4 Monoclonal antibody (66350-1-Ig)

    WB analysis of rat liver using 66350-1-Ig (same clone as 66350-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using TLR4 Monoclonal antibody (66350-1-Ig)

    WB analysis of NIH/3T3 using 66350-1-Ig (same clone as 66350-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66350-1-Ig (TLR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TLR4 Monoclonal antibody (66350-1-Ig)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TLR4 Monoclonal antibody (66350-1-Ig)

    IHC staining of human tonsillitis using 66350-1-Ig (same clone as 66350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66350-1-Ig (TLR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66350-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TLR4 Monoclonal antibody, PBS Only

TLR4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66350-1-PBS
Clone No.3G9A4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

3G9A4, ARMD10, CD284, hToll, TOLL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66350-1-PBS targets TLR4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag13857

Product name: Recombinant human TLR4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 653-839 aa of BC117422

Sequence: KFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI

相同性解析による交差性が予測される生物種
Full Name toll-like receptor 4
Calculated molecular weight 839 aa, 96 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC117422
Gene Symbol TLR4
Gene ID (NCBI) 7099
RRIDAB_2881730
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00206
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TLR4, also named CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.