Various lysates were subjected to SDS PAGE followed by western blot with 15240-1-AP (HLA class I ABC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP result of anti-HLA class I ABC (IP:15240-1-AP, 3ug; Detection:15240-1-AP 1:500) with HepG2 cells lysate 1720ug.
1X10^6 HepG2 cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (15240-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed .
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
