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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HLA-A Polyclonal antibody

HLA-A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55383-1-AP

Synonyms

HLA A, HLAA, HLA-A, MHC class I antigen A*3

Validation Data Gallery

Applications:
  • WB analysis using 55383-1-AP

    WB analysis using 55383-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55383-1-AP (HLA-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 55383-1-AP

    WB analysis of HeLa using 55383-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55383-1-AP (HLA-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55383-1-AP

    IHC staining of human kidney using 55383-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 55383-1-AP

    IHC staining of human kidney using 55383-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 55383-1-AP

    IHC staining of human small intestine using 55383-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 55383-1-AP

    IHC staining of human small intestine using 55383-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, A431 cells
Positive IHC detected inhuman kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

55383-1-AP targets HLA-A in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 41-44 kDa
GenBank accession numberNM_002116
Gene symbol HLA-A
Gene ID (NCBI) 3105
RRIDAB_10888639
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
WB protocol for HLA-A antibody 55383-1-APDownload protocol
IHC protocol for HLA-A antibody 55383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Virol

Two Functional Variants of AP-1 Complexes Composed of either γ2 or γ1 Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 Nef.

Authors - Lucas A Tavares
View Article