Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15240-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15240-1-AP (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 15240-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15240-1-AP (HLA class I ABC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15240-1-AP
IP result of anti-HLA class I ABC (IP:15240-1-AP, 4ug; Detection:15240-1-AP 1:20000) with HepG2 cells lysate 2000 ug.
IHC staining of human tonsillitis using 15240-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 15240-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 15240-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 15240-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15240-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 15240-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of Raji using 15240-1-AP
Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HLA class I ABC Polyclonal antibody

HLA class I ABC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15240-1-AP

Publications(64)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA, Cell treatment

Synonyms

HLA-A, HLA A, HLA class I, HLA class I (HLA-A), HLA class I histocompatibility antigen, A alpha chain

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (64)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, NCI-H1299 cells, Calu-1 cells, HEK-293 cells, HeLa cells, HepG2 cells, L02 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human tonsillitis tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 36 publications below
IHC	See 20 publications below
IF	See 16 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

15240-1-AP targets HLA class I ABC in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7370 Product name: Recombinant human HLA class I (HLA-A) protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-303 aa of BC003069 Sequence: SMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQ 相同性解析による交差性が予測される生物種
Full Name	major histocompatibility complex, class I, A
Calculated molecular weight	41 kDa
Observed molecular weight	44 kDa
GenBank accession number	BC003069
Gene Symbol	HLA-A
Gene ID (NCBI)	3105
RRID	AB_1557426
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P04439
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
IF protocol for HLA class I ABC antibody 15240-1-AP	Download protocol
IHC protocol for HLA class I ABC antibody 15240-1-AP	Download protocol
IP protocol for HLA class I ABC antibody 15240-1-AP	Download protocol
WB protocol for HLA class I ABC antibody 15240-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion Authors - Lina Herhaus View Article
human	WB	Nature Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer. Authors - Xinjian Liu View Article
human	WB	Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis Authors - Chuan Yang View Article
human	WB	Cell Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy. Authors - Kazuhiro Koikawa View Article
human	IHC	Cancer Discov Spatial-Temporal Diversity of Extrachromosomal DNA Shapes Urothelial Carcinoma Evolution and the Tumor Immune Microenvironment Authors - Wei Lv View Article
human		Nat Cell Biol An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles. Authors - James W Clancy View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15240-1-AP

WB analysis using 15240-1-AP

IP experiment of HepG2 using 15240-1-AP

IHC staining of human tonsillitis using 15240-1-AP

IHC staining of human tonsillitis using 15240-1-AP

IHC staining of human stomach cancer using 15240-1-AP

IHC staining of human stomach cancer using 15240-1-AP

IF Staining of HepG2 using 15240-1-AP

IF Staining of HepG2 using 15240-1-AP

IF Staining of Raji using 15240-1-AP

HLA class I ABC Polyclonal antibody

HLA class I ABC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion

Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.

Spatial-Temporal Diversity of Extrachromosomal DNA Shapes Urothelial Carcinoma Evolution and the Tumor Immune Microenvironment

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.