Validation Data Gallery
Product Information
The ChromoTek mEos-Trap Magnetic Agarose consists of an anti-mEos Nanobody/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of proteins fused to the mEos-tag from cell extracts of various organisms.
| Description | Immunoprecipitation of mEos-fusion proteins with anti-mEos Nanobody conjugated to magnetic agarose beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to members of the mEos-tag family fused to a protein of interest at N-, C- or internal position. To ensure broad coverage of mEos variants, the Nanobody was optimized for binding to both mEos2 and mEos4b, which are representative of the different lineages of the mEos family. There is no cross-reactivity to other fluorescent proteins such as eGFP. |
| Binding Capacity | 10 ug of recombinant mEos4b (28 kDa) per 25 ul bead slurry |
| Conjugate | Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5 |
| Wash Buffer Compatibility | 2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.2% SDS, 1M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Camelid |
| Affinity (KD) | 75 nM |
| Compatibility with mass spectrometry | The mEos-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3552229 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
|---|
| metma_SDS_mEos-Trap Magnetic Agarose (EN) |
| Datasheet |
|---|
| Protocol mEos-Trap Magnetic Agarose (PDF) |