Validation Data Gallery
Product Information
The ChromoTek mEos-Trap Agarose Kit consists of an anti-mEos Nanobody/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mEos-tagged proteins from cell extracts of various organisms.
| Description | The HA-Trap Agarose Kit contains mEos-Trap agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of mEos-tagged proteins. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to members of the mEos-tag family fused to a protein of interest at N-, C- or internal position. To ensure broad coverage of mEos variants, the Nanobody was optimized for binding to both mEos2 and mEos4b, which are representative of the different lineages of the mEos family. There is no cross-reactivity to other fluorescent proteins such as eGFP. |
| Binding Capacity | 15 ug of recombinant mEos4b (28 kDa) per 25 uL bead slurry |
| Conjugate | Agarose beads; ~90 um (cross-linked 4% agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lammli) |
| Wash Buffer Compatibility | 2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.2% SDS, 1 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Camelid |
| Affinity (KD) | 75 nM |
| Compatibility with mass spectrometry | The mEos-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3552227 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze! |
Kit components
| Component | Description |
|---|---|
| mEos-Trap Agarose | 20 reactions (500 µl) |
| Lysis buffer | Optimized for cytoplasmic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |