Validation Data Gallery

  • Co-IP of BAX and BAK using anti-BAX IgGs from different vendors using anti-ms / anti-rb IgG Magnetic VHH Agarose (mrIGma). Tested IgGs include such of rabbit and mouse origin with IgG1 and IgG2b subtypes. 5 µg of respective IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of Input (IN) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western Blot analysis BAX was detected using a polyclonal rb IgG (PTG: 50599-2-Ig) (1:2000) labeled using the FlexAble HRP Antibody Labeling Kit for rabbit IgG. This allows a clean detection of BAX, without interfering signals of IgG heavy and light chains derived from the spiked rabbit antibodies. The presence of BAK co-precipitated with BAX was tested using a polyclonal  rb IgG (PTG 29552-1-AP) 1:2000, labeled with FlexAble HRP. While all IgGs allowed for efficient IP of BAX, BAK was absent in the case of the primary antibody from vendor D, possibly due to the BAX:BAK interaction interface being masked by this particular IgG.

    Co-IP of BAX and BAK using anti-BAX IgGs from different vendors using anti-ms / anti-rb IgG Magnetic VHH Agarose (mrIGma). Tested IgGs include such of rabbit and mouse origin with IgG1 and IgG2b subtypes. 5 µg of respective IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of Input (IN) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western Blot analysis BAX was detected using a polyclonal rb IgG (PTG: 50599-2-Ig) (1:2000) labeled using the FlexAble HRP Antibody Labeling Kit for rabbit IgG. This allows a clean detection of BAX, without interfering signals of IgG heavy and light chains derived from the spiked rabbit antibodies. The presence of BAK co-precipitated with BAX was tested using a polyclonal  rb IgG (PTG 29552-1-AP) 1:2000, labeled with FlexAble HRP. While all IgGs allowed for efficient IP of BAX, BAK was absent in the case of the primary antibody from vendor D, possibly due to the BAX:BAK interaction interface being masked by this particular IgG.

ChromoTek anti-Rabbit IgG / anti-Mouse IgG Magnetic VHH Agarose for Immunoprecipitation

anti-Rabbit / anti-Mouse IgG IP Beads is an affinity resin for IP of all subtypes of Rabbit and Mouse IgG. It consists of rabbit and mouse IgG specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.
Cat No. mrIGma

Reactivity

Rabbit IgG / Mouse IgG

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Magnetic Agarose

Mouse IP, Rabbit IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody

Formats:  Magnetic Agarose
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Product Information

anti-Rabbit / anti-Mouse IgG IP Beads is an affinity resin for IP of all subtypes of Rabbit and Mouse IgG. It consists of rabbit and mouse IgG specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.

DescriptionReady to use VHH coupled Magnetic Agarose Beads for Immunoprecipitation of all subtypes of Rabbit and Mouse IgG.

• Fast, reliable and efficient one-step Immunoprecipitation

• Ready-to-use

• Stable in harsh buffer conditions

• Suitable for downstream mass spec analysis

• Specific to all Rabbit and all Mouse IgG subtypes

• Low background

ApplicationsIP, Co-IP
Binding capacity/
ConjugateMagnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
Elution bufferSDS Sample Buffer
Wash buffer compatibility/
TypeNanobody
ClassRecombinant - Animal free production
HostAlpaca
Affinity (KD)/
ImmunogenRabbit IgG / Mouse IgG
RRIDAB_3697101
Storage Buffer20% Ethanol
Storage Condition+4°C / do not freeze!
ShippingAmbient Temp
Size1 ml; 2 ml; 5 ml