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Validation Data Gallery

  • HeLa cells transiently transfected with human CD16 were immunostained with anti-CD16 VHH-FITC (code, 1:500, green). Nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed. Note: Immunostaining with anti-CD16-VHH-Dye can be performed live or after formaldehyde fixation. 

    HeLa cells transiently transfected with human CD16 were immunostained with anti-CD16 VHH-FITC (code, 1:500, green). Nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed. Note: Immunostaining with anti-CD16-VHH-Dye can be performed live or after formaldehyde fixation. 

  • Live human PBMCs were gravity-sedimented and immunostained with anti-CD16 recombinant VHH, FITC Plus (FITC-CD16, green) and anti-CD3 IgG-CL555 (CL555-65151, magenta). Images were acquired with a 20x objective and post-processed. Dead cells (autofluorescent in all channels including UV and far-red) we subtracted from all channels with ImageJ. Note: Immunostaining with FITC-CD16 can be performed live or after formaldehyde fixation. 

    Live human PBMCs were gravity-sedimented and immunostained with anti-CD16 recombinant VHH, FITC Plus (FITC-CD16, green) and anti-CD3 IgG-CL555 (CL555-65151, magenta). Images were acquired with a 20x objective and post-processed. Dead cells (autofluorescent in all channels including UV and far-red) we subtracted from all channels with ImageJ. Note: Immunostaining with FITC-CD16 can be performed live or after formaldehyde fixation. 

ChromoTek anti-CD16 recombinant VHH, FITC Plus

FITC-CD16 is a recombinant FITC Plus conjugated anti CD16 Nanobody (VHH) that targets IL6 in IF and FC applications, showing reactivity with human samples.
Cat No. FITC-CD16

Host/Type

Alpaca VHH

Reactivity

Human

Applications

IF, FC

Conjugate

FITC Plus

Synonyms

CD16 VHH, CD16 single domain antibody (sdAb), FCGR3, IGFR3, Fc-gamma R3, FcRIII

Formats:  FITC Plus
Size/Concentration: 

-/ -

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Product Information

FITC-CD16 is a recombinant FITC Plus conjugated anti CD16 Nanobody (VHH) that targets IL6 in IF and FC applications, showing reactivity with human samples.

ApplicationsIF, FC
ReactivityHuman
ConjugateFITC Plus
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity2 nM for CD16A, 4 nM for CD16B
Molecular Weight15.6 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths500 nm / 520 nm
RRIDAB_3665974
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingblue ice
BackgroundCD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.