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Species Reactivity

Species Reactivity

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Validation Data Gallery

  • HeLa cells transiently transfected with human CD16 were immunostained with anti-CD16 recombinant VHH, CoraLite ® Plus 647 (CL647-CD16, 1:500, magenta). Nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed. Note: Immunostaining with CL647-CD16 can be performed live or after formaldehyde fixation.

    HeLa cells transiently transfected with human CD16 were immunostained with anti-CD16 recombinant VHH, CoraLite ® Plus 647 (CL647-CD16, 1:500, magenta). Nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 20x objective and post-processed. Note: Immunostaining with CL647-CD16 can be performed live or after formaldehyde fixation.

  • Live human PBMCs were gravity-sedimented and immunostained with anti-CD16 recombinant VHH, CoraLite ® Plus 647 (CL647-CD16, magenta) and anti-CD3 IgG-CL488 (CL488-65151, green). Images were acquired with a 20x objective and post-processed. Dead cells (autofluorescent in all channels including UV and far-red) we subtracted from all channels with ImageJ. Note: Immunostaining with CL647-CD16 can be performed live or after formaldehyde fixation.

    Live human PBMCs were gravity-sedimented and immunostained with anti-CD16 recombinant VHH, CoraLite ® Plus 647 (CL647-CD16, magenta) and anti-CD3 IgG-CL488 (CL488-65151, green). Images were acquired with a 20x objective and post-processed. Dead cells (autofluorescent in all channels including UV and far-red) we subtracted from all channels with ImageJ. Note: Immunostaining with CL647-CD16 can be performed live or after formaldehyde fixation.

ChromoTek anti-CD16 recombinant VHH, CoraLite ® Plus 647

CL647-CD16 is a recombinant CoraLite ® Plus 647 conjugated anti CD16 Nanobody (VHH) that targets IL6 in IF and FC applications, showing reactivity with human samples.
Cat No. CL647-CD16

Host/Type

Alpaca VHH

Reactivity

Human

Applications

IF, FC

Conjugate

CoraLite® Plus 647

Synonyms

CD16 VHH, CD16 single domain antibody (sdAb), FCGR3, IGFR3, Fc-gamma R3, FcRIII

Formats:  CoraLite ® Plus 647
Size/Concentration: 

-/ -

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Product Information

CL647-CD16 is a recombinant CoraLite ® Plus 647 conjugated anti CD16 Nanobody (VHH) that targets IL6 in IF and FC applications, showing reactivity with human samples.

ApplicationsIF, FC
ReactivityHuman
ConjugateCoraLite® Plus 647
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity2 nM for CD16A, 4 nM for CD16B
Molecular Weight15.6 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
RRIDAB_3665404
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingblue ice
BackgroundCD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.