ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 [CTK0101, CTK0102]
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.
Cat no : srbAF647-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.
Applications | IF, WB, FC |
Recommended Dilution | Immunofluorescence 1:1,000 Super-resolution microscopy 1:1,000 Western blot 1:1,000 Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000. Note: Image acquisition time may have to be optimized |
Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
Host | Alpaca |
Affinity | CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM |
Specificity/Target | Human IgG and rabbit IgG |
Reactivity | Rabbit, Human, Macaque |
Conjugate | Alexa Fluor® 647 |
Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
Documentation
SDS |
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SDS_Nano-Secondary® alpaca anti-human IgG, anti-rabbit IgG, Alexa Fluor® 647 |
Protocol |
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Protocol Nano-Secondary® alpaca anti-human IgG, anti-rabbit IgG, Alexa Fluor® 647 |
Application note |
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One-step incubation with Nano-Secondaries (PDF) |
Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
Brochure |
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Chromotek nanobodies brochure (PDF) |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Joe (Verified Customer) (04-26-2022) | I used this secondary in an indirect flow cytometry experiment, staining for a phospho-protein. I found that this secondary resulted in a stronger signal when compared to my regular life scientific alexa fluor secondary antibody. I will be using these nanobodies from now on! The antibody was used at a dilution of 1:4000, 30m RT staining.
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