ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 [CTK0101, CTK0102]

Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.

Applications

IF, WB, FC

Conjugate

Alexa Fluor® 647

Host

Alpaca

Reactivity

Rabbit, Human, Macaque

Cat no : srbAF647-1

Synonyms

human IgG, rabbit IgG, immunoglobulin Gamma



Product Information

Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH reagent uses a novel class of anti-rabbit IgG and anti-human IgG specific antibodies. This secondary antibody product consists of Nanobodies that bind to rabbit IgG and human IgG with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
ApplicationsIF, WB, FC
HostAlpaca
Species ReactivityRabbit, Human, Macaque
No cross-reactivity to mouse, rat, sheep, goat, and guinea pig IgG
ConjugateAlexa Fluor® 647
Physical StateLiquid
Suggested DilutionImmunofluorescence 1:1,000
Super-resolution microscopy 1:1,000
Western blot 1:1,000
Purification MethodRecombinant expression, affinity purification IMAC
TypeMixture of 2 monoclonal Nanobodies
ClassRecombinant
Affinity (KD) CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionStore at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
RRIDAB_2827587
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Biosens Bioelectron

3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing

Authors - Xiaoyan Lyu
IF

Nat Methods

Spatial single-cell mass spectrometry defines zonation of the hepatocyte proteome

Authors - Florian A Rosenberger
IF

Vet Microbiol

NDV inhibited IFN-β secretion through impeding CHCHD10-mediated mitochondrial fusion to promote viral proliferation

Authors - Xibing Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Clarisse (Verified Customer) (08-08-2022)

This secondary nano-body performs incredibly well in both single-step and sequential IF incubations. The dilution used was 1:1000. The signal is way more specific compared to conventional alexa secondaries. You should definitely give it a try!

  • Applications: Immunofluorescence
  • Cell Tissue Type: Human cerebral organoids
FH

Joe (Verified Customer) (04-26-2022)

I used this secondary in an indirect flow cytometry experiment, staining for a phospho-protein. I found that this secondary resulted in a stronger signal when compared to my regular life scientific alexa fluor secondary antibody. I will be using these nanobodies from now on! The antibody was used at a dilution of 1:4000, 30m RT staining.

  • Applications: Flow Cytometry
  • Cell Tissue Type: K562