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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated XRCC2 Monoclonal antibody

XRCC2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D9A1

Cat no : CL594-66652

Synonyms

DKFZp781P0919, DNA repair protein XRCC2, XRCC2

Validation Data Gallery

Applications:
  • IF Staining of L02 using CL594-66652

    IF Staining of L02 using CL594-66652

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using CoraLite®594-conjugated XRCC2 antibody (CL594-66652, Clone: 3D9A1 ) at dilution of 1:100, CL488-Phalloidin (green).

  • IF Staining of HepG2 using CL594-66652

    IF Staining of HepG2 using CL594-66652

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66652 (XRCC2 antibody) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-66652

    FC experiment of HepG2 using CL594-66652

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human XRCC2 (CL594-66652, Clone:3D9A1) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inL02 cells, HepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66652-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66652 targets XRCC2 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC2 fusion protein Ag14421 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 2
Calculated molecular weight 280 aa, 32 kDa
GenBank accession numberBC042137
Gene symbol XRCC2
Gene ID (NCBI) 7516
RRIDAB_2883610
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

Protocols

Product Specific Protocols
IF protocol for CL594 XRCC2 antibody CL594-66652Download protocol
FC protocol for CL594 XRCC2 antibody CL594-66652Download protocol
Standard Protocols
Click here to view our Standard Protocols