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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    WB analysis of HEK-293 using 66652-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    WB analysis of Raji using 66652-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    WB analysis of NIH/3T3 using 66652-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    IF Staining of HepG2 using 66652-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    IF Staining of HepG2 using 66652-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    FC experiment of HepG2 using 66652-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRCC2 (66652-1-Ig, Clone:3D9A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

XRCC2 Monoclonal antibody

XRCC2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66652-1-Ig
Clone No.3D9A1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

3D9A1, DNA repair protein XRCC2, X-ray repair cross-complementing protein 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Raji cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66652-1-Ig targets XRCC2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14421

Product name: Recombinant human XRCC2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-280 aa of BC042137

Sequence: MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC

相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 2
Calculated molecular weight 280 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC042137
Gene Symbol XRCC2
Gene ID (NCBI) 7516
RRIDAB_2882009
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

Protocols

Product Specific Protocols
IF protocol for XRCC2 antibody 66652-1-IgDownload protocol
WB protocol for XRCC2 antibody 66652-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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