V5-Trap™ Magnetic Agarose

The ChromoTek V5-Trap™ Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

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(希望販売価格)

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カタログ番号:
v5tma

Applications:
IP, CoIP, ChIP, RIP

Clonality:
Monoclonal

Host:
Alpaca

Affinity:
Dissociation constant KD of 40 nM

Specificity/Target:
V5-tag amino acid sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.

Coupled VHH:
recombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment

Conjugate:
Magnetic Agarose

Beads:
~ 40 µm

Binding capacity:
10 µL slurry bind ~ 7 µg of recombinant V5-tagged protein ~30 kDa

Elution buffer:
V5-peptide. For details see below.
SDS sample buffer
0.1 M glycine pH 2.0

Storage Buffer:
20% EtOH

Storage Condition:
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Synonyms:
V5 tag, V5, GKPIPNPLLGLDST, V5-Trap

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