Validation Data Gallery

  • V5-Trap® Magnetic Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-through, B: Bound.

    V5-Trap® Magnetic Agarose for immunoprecipitation of V5-tagged proteins. The V5-tag is C-terminal. I: Input, FT: Flow-through, B: Bound.

  • Pulldown of V5-tagged protein (V5-tag at C-terminus) with V5-Trap® Magnetic Agarose beads, Coomassie and Western blot. Western blot: V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse IgG1 Nano-Secondary Alexa Fluor® 488 I: input, FT: Flow-through, B: Bound.

    Pulldown of V5-tagged protein (V5-tag at C-terminus) with V5-Trap® Magnetic Agarose beads, Coomassie and Western blot. Western blot: V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and anti-mouse IgG1 Nano-Secondary Alexa Fluor® 488 I: input, FT: Flow-through, B: Bound.

  • V5-Trap® Magnetic Agarose for pull-down of V5-tagged proteins. V5 at the N-terminus. I: Input, FT: Flow-through, B: Bound.

    V5-Trap® Magnetic Agarose for pull-down of V5-tagged proteins. V5 at the N-terminus. I: Input, FT: Flow-through, B: Bound.

  • Very low background of V5-Trap® Magnetic Agarose: IP from different cell lysates without V5-tagged protein.

    Very low background of V5-Trap® Magnetic Agarose: IP from different cell lysates without V5-tagged protein.

ChromoTek V5-Trap® Magnetic Agarose

The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.
Cat No. v5tma

Specificity

V5-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic agarose

V5 tag, V5, GKPIPNPLLGLDST, V5-Trap

Size/Concentration: 

-/ -


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Product Information

The ChromoTek V5-Trap® Magnetic Agarose is an affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.

DescriptionImmunoprecipitation of V5-tagged proteins and their interacting factors with anti-V5 Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetV5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Binding capacity17.5 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferV5-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 40 nM
Compatibility with mass spectrometryThe V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2868498
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
Size25ul/reactions (eg:20rxns=500ul slurry)

Publications

ApplicationTitle

Cell

SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9

Authors - Nora Schmidt
IP

Cell

A conserved fertilization complex bridges sperm and egg in vertebrates

Authors - Victoria E Deneke
IP

Nat Cell Biol

Noncanonical inheritance of phenotypic information by protein amyloids

Authors - Matthew Eroglu

Neuron

Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome

Authors - Piero Sanfilippo
IP

Mol Cell

Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants

Authors - Chao Yang
IP-MS

Proc Natl Acad Sci U S A

Disproportionate investment in Spiralin B production limits in-host growth and favors the vertical transmission of Spiroplasma insect endosymbionts.

Authors - Florent Masson