Validation Data Gallery
![V5-Trap® Agarose for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 488 I: Input, FT: Flow-Through, B: Bound.](https://www.ptglab.com/products/pictures/Result-v5ta_20220118093523852.png "V5-Trap® Agarose for immunoprecipitation of V5 fusion proteins. HEK293T cell lysate with V5-tagged protein. Coomassie and Western blot. V5-tag antibody [SV5-P-K], monoclonal mouse IgG1 kappa and Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor® 488 I: Input, FT: Flow-Through, B: Bound.")
Product Information
V5-Trap® Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.
| Description | Immunoprecipitation of V5-tagged proteins and their interacting factors with anti-V5 Nanobody conjugated to beads.
• No heavy & light antibody chains in downstream applications • Very low background • Effective elution with V5-peptide • Stable under harsh washing conditions • Efficient immunoprecipitation of V5-tag fusion proteins |
| Applications | IP, CoIP, ChIP, RIP, Protein purification |
| Specificity/Target | V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein. |
| Binding capacity | 15 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | V5-peptide SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 40 nM |
| Compatibility with mass spectrometry | The V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2868497 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
|---|
| v5ta_SDS_V5-Trap® Agarose (EN) |
| Datasheet |
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| Protocol V5-Trap Agarose (PDF) |
| Troubleshooting |
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| Troubleshooting guide immunoprecipitation (PDF) |
Publications
| Application | Title |
|---|---|
Cell PINK1, Keap1, and Rtnl1 regulate selective clearance of endoplasmic reticulum during development | |
Sci Adv Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2 | |
Mol Biol Evol Duplication of horizontally acquired GH5_2 enzymes played a central role in the evolution of longhorned beetles. | |
Mol Cell Proteomics A Proteomic Study on the Membrane Protein Fraction of T Cells Confirms High Substrate Selectivity for the ER Translocation Inhibitor Cyclotriazadisulfonamide. | |
J Chromatogr A An engineered peptide tag-specific nanobody for immunoaffinity chromatography application enabling efficient product recovery at mild conditions. | |
Mol Microbiol Kinetoplastid-specific X2-family kinesins interact with a kinesin-like pleckstrin homology domain protein that localizes to the trypanosomal microtubule quartet. |