Validation Data Gallery
Product Information
The Ubiquitin-Trap Magnetic Agarose Kit is a ready-to-use reagent for the IP of ubiquitin and ubiquitinylated proteins. It consists of an anti-ubiquitin Nanobody/VHH coupled to magnetic agarose beads, along with lysis, wash, and elution buffers to use in the IP process.
| Description | The Ubiquitin-Trap Magnetic Agarose Kit contains Ubiquitin-Trap magnetic agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of ubiquitin and ubiqutinylated proteins. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, CoIP |
| Specificity/Target | Binds to monomeric ubiquitin, ubiquitin chains, and ubiquitinylated proteins. Ubiquitin chains may be linked via lysines; linkages via lysines such as K11, K48 and K63 are compatible with binding by the Ubiquitin-Trap (further linkage types also likely to be compatible, but not tested). |
| Tested Reactivity | Human, Mouse, Hamster, Dog, Spinach, Yeast |
| Conjugate | Magnetic agarose beads; ~40 um (cross-linked 6% magnetic agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lammli) |
| Wash Buffer Compatibility | 2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Camelid |
| Affinity | The KD for monomeric ubiquitin is 90 nM. The apparent affinity is even higher for ubiquitin chains due to avidity effects. |
| Compatibility with mass spectrometry | The Ubiquitin-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2943578 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze! |
Kit components
| Component | Description |
|---|---|
| Ubiquitin-Trap Agarose | 20 reactions (500 µl) |
| Lysis buffer | Optimized for cytoplasmic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| utmak_SDS_Ubiquitin-Trap Magnetic Agarose Kit (EN) |
| Datasheet |
|---|
| Datasheet_Ubiquitin-Trap Magnetic Agarose Kit(PDF) |