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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TOP2A Polyclonal antibody

TOP2A Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24641

Synonyms

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL488-24641

    IF Staining of A431 using CL488-24641

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 TOP2A antibody (CL488-24641) at dilution of 1:400.

Tested Applications

Positive IF detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 24641-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-24641 targets TOP2A in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOP2A fusion protein Ag19551 相同性解析による交差性が予測される生物種
Full Name topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight 1531 aa, 174 kDa
Observed molecular weight 174 kDa
GenBank accession numberBC140791
Gene symbol TOP2A
Gene ID (NCBI) 7153
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TOP2A antibody CL488-24641Download protocol
Standard Protocols
Click here to view our Standard Protocols