Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 using 24641-1-AP
WB result of TOP2A antibody (24641-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells.
WB analysis using 24641-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 24641-1-AP
WB result of TOP2A antibody (24641-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells.
WB analysis of HeLa using 24641-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24641-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 24641-1-AP
IP result of anti-TOP2A (IP:24641-1-AP, 4ug; Detection:24641-1-AP 1:1000) with HeLa cells lysate 2000ug.
IHC staining of human pancreas cancer using 24641-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 24641-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24641-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 24641-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.
IF Staining of A431 using 24641-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (24641-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

TOP2A Polyclonal antibody

TOP2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24641-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II, TOP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (26)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, HeLa cells, HepG2 cells, MDA-MB-231 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 5 publications below
IF	See 5 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

24641-1-AP targets TOP2A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19551 Product name: Recombinant human TOP2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1187-1531 aa of BC140791 Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF 相同性解析による交差性が予測される生物種
Full Name	topoisomerase (DNA) II alpha 170kDa
Calculated molecular weight	1531 aa, 174 kDa
Observed molecular weight	174 kDa
GenBank accession number	BC140791
Gene Symbol	TOP2A
Gene ID (NCBI)	7153
RRID	AB_2879651
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11388
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Protocols

Product Specific Protocols
IF protocol for TOP2A antibody 24641-1-AP	Download protocol
IHC protocol for TOP2A antibody 24641-1-AP	Download protocol
IP protocol for TOP2A antibody 24641-1-AP	Download protocol
WB protocol for TOP2A antibody 24641-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Comparative proteomic landscapes elucidate human preimplantation development and failure Authors - Wencheng Zhu View Article
human	WB	Cell Mol Biol Lett STUB1 downregulates TOP2A through a dual mechanism of ubiquitination and FOXM1-mediated transcription repression, suppressing breast cancer growth and enhancing sensitivity to chemotherapy. Authors - Baohui Yue View Article
mouse	IHC	Cell Death Discov Kinase activity profiling identifies putative downstream targets of cGMP/PKG signaling in inherited retinal neurodegeneration. Authors - Akanksha Roy View Article
human	WB	Cell Chem Biol Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation Authors - Adil Muneer View Article
rabbit,human	IF	Stem Cell Res Ther A single-cell transcriptomic atlas of human lens epithelium: identification and functional insights into lens stem/progenitor cells Authors - Yuzhou Gu View Article
		J Ethnopharmacol Yang-Xue-An-Tai Decoction Treats URSA by regulating the differentiation of proliferating decidual stromal cells through multiple targets. Authors - Guanshan Li View Article

Validation Data Gallery

WB analysis of HepG2 using 24641-1-AP

WB analysis using 24641-1-AP

WB analysis of HepG2 using 24641-1-AP

WB analysis of HeLa using 24641-1-AP

WB analysis of HeLa using 24641-1-AP

IP experiment of HeLa using 24641-1-AP

IHC staining of human pancreas cancer using 24641-1-AP

IHC staining of human pancreas cancer using 24641-1-AP

IHC staining of human breast cancer using 24641-1-AP

IHC staining of human breast cancer using 24641-1-AP

IF Staining of A431 using 24641-1-AP

TOP2A Polyclonal antibody

TOP2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Comparative proteomic landscapes elucidate human preimplantation development and failure

STUB1 downregulates TOP2A through a dual mechanism of ubiquitination and FOXM1-mediated transcription repression, suppressing breast cancer growth and enhancing sensitivity to chemotherapy.

Kinase activity profiling identifies putative downstream targets of cGMP/PKG signaling in inherited retinal neurodegeneration.

Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation

A single-cell transcriptomic atlas of human lens epithelium: identification and functional insights into lens stem/progenitor cells

Yang-Xue-An-Tai Decoction Treats URSA by regulating the differentiation of proliferating decidual stromal cells through multiple targets.

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