- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated TLE1 Monoclonal antibody
TLE1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F2H11
Cat no : CL488-66216
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66216 targets TLE1 in IF applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TLE1 fusion protein Ag21327 相同性解析による交差性が予測される生物種 |
Full Name | transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) |
Calculated molecular weight | 83 kDa |
Observed molecular weight | 85-95 kDa |
GenBank accession number | BC010100 |
Gene symbol | TLE1 |
Gene ID (NCBI) | 7088 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transducin-like enhancer protein 1(TLE1) is a transcriptional corepressor. It can bind to and regress expression of many transcription factors such as NFkB, which is mediated by FOXA2, CTNNB1 and TCF family member in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Expression of TLE1 is highest in skeletal muscle and liver, moderate in placenta, heart, kidney, and spleen, and weak in all other tissues including brain.
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 TLE1 antibody CL488-66216 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |