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  • Featured Product
  • KD/KO Validated

TLE1 Monoclonal antibody

TLE1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F2H11

Cat no : 66216-1-Ig

Synonyms

E(Sp1) homolog, ESG, ESG1, GRG1, TLE1

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 66216-1-Ig

    WB analysis of HEK-293 using 66216-1-Ig

    WB result of TLE1 antibody (66216-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLE1 transfected HEK-293 cells.

  • WB analysis of Jurkat using 66216-1-Ig

    WB analysis of Jurkat using 66216-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66216-1-Ig (TLE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 66216-1-Ig

    IHC staining of human gliomas using 66216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 66216-1-Ig

    IHC staining of human gliomas using 66216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66216-1-Ig (TLE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using 66216-1-Ig

    FC experiment of Jurkat using 66216-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human TLE1 (66216-1-Ig, Clone:2F2H11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (black). Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 750 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66216-1-Ig targets TLE1 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLE1 fusion protein Ag21327 相同性解析による交差性が予測される生物種
Full Name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Calculated molecular weight 83 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC010100
Gene symbol TLE1
Gene ID (NCBI) 7088
RRIDAB_2881607
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transducin-like enhancer protein 1(TLE1) is a transcriptional corepressor. It can bind to and regress expression of many transcription factors such as NFkB, which is mediated by FOXA2, CTNNB1 and TCF family member in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Expression of TLE1 is highest in skeletal muscle and liver, moderate in placenta, heart, kidney, and spleen, and weak in all other tissues including brain.

Protocols

Product Specific Protocols
WB protocol for TLE1 antibody 66216-1-IgDownload protocol
IHC protocol for TLE1 antibody 66216-1-IgDownload protocol
FC protocol for TLE1 antibody 66216-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols