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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated TEX14 Monoclonal antibody

TEX14 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A11D6

Cat no : CL594-67982

Synonyms

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

Validation Data Gallery

Applications:
  • IF Staining of mouse testis using CL594-67982

    IF Staining of mouse testis using CL594-67982

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 TEX14 antibody (CL594-67982, Clone: 1A11D6 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-67982

    IF Staining of mouse testis using CL594-67982

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 TEX14 antibody (CL594-67982, Clone: 1A11D6 ) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 67982-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67982 targets TEX14 in IF applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TEX14 fusion protein Ag13143 相同性解析による交差性が予測される生物種
Full Name testis expressed 14
Calculated molecular weight957aa,107 kDa; 1497aa,168 kDa
Observed molecular weight180 kDa
GenBank accession numberBC040526
Gene symbol TEX14
Gene ID (NCBI) 56155
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.

Protocols

Product Specific Protocols
IF protocol for CL594 TEX14 antibody CL594-67982Download protocol
Standard Protocols
Click here to view our Standard Protocols