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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TEX14 Monoclonal antibody

TEX14 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A11D6

Cat no : 67982-1-Ig

Synonyms

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

Validation Data Gallery

Applications:
  • WB analysis of rat testis using 67982-1-Ig

    WB analysis of rat testis using 67982-1-Ig

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 67982-1-Ig

    WB analysis of mouse testis using 67982-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit testis using 67982-1-Ig

    WB analysis of rabbit testis using 67982-1-Ig

    rabbit testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 67982-1-Ig

    WB analysis of PC-3 using 67982-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 67982-1-Ig

    IF Staining of mouse testis using 67982-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse testis using 67982-1-Ig

    IF Staining of mouse testis using 67982-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inrat testis tissue, PC-3 cells, mouse testis tissue, rabbit testis tissue
Positive IF detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67982-1-Ig targets TEX14 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TEX14 fusion protein Ag13143 相同性解析による交差性が予測される生物種
Full Name testis expressed 14
Calculated molecular weight957aa,107 kDa; 1497aa,168 kDa
Observed molecular weight180 kDa
GenBank accession numberBC040526
Gene symbol TEX14
Gene ID (NCBI) 56155
RRIDAB_2918731
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.

Protocols

Product Specific Protocols
WB protocol for TEX14 antibody 67982-1-IgDownload protocol
IF protocol for TEX14 antibody 67982-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols