Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-67140 targets TCF3 in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15734 Product name: Recombinant human TCF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 318-480 aa of BC110580 Sequence: WPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLPGHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTRCQ 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) |
| Calculated molecular weight | 654 aa, 68 kDa |
| Observed molecular weight | 68-70 kDa |
| GenBank accession number | BC110580 |
| Gene Symbol | TCF3 |
| Gene ID (NCBI) | 6929 |
| RRID | AB_2883640 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15923 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 TCF3 antibody CL594-67140 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
