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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis using 67140-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of PC-3 using 67140-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of Jurkat using 67140-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of K-562 using 67140-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of HepG2 using 67140-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of NCI-H1299 using 67140-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of PC-3 using 67140-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of Jurkat using 67140-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of K-562 using 67140-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    WB analysis of THP-1 using 67140-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    IF Staining of HepG2 using 67140-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TCF3 antibody (67140-1-Ig, Clone: 1D12E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TCF3 Monoclonal antibody (67140-1-Ig)

    IF Staining of HepG2 using 67140-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67140-1-Ig (TCF3 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO Validated

TCF3 Monoclonal antibody

TCF3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67140-1-Ig
Clone No.1D12E5
Publications(3)

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, NCI-H1299 cells, PC-3 cells, Jurkat cells, K-562 cells, THP-1 cells, A549 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67140-1-Ig targets TCF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag15734

Product name: Recombinant human TCF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 318-480 aa of BC110580

Sequence: WPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLPGHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTRCQ

相同性解析による交差性が予測される生物種
Full Name transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Calculated molecular weight 654 aa, 68 kDa
Observed molecular weight 68-70 kDa
GenBank accession numberBC110580
Gene Symbol TCF3
Gene ID (NCBI) 6929
RRIDAB_2882439
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP15923
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for TCF3 antibody 67140-1-IgDownload protocol
WB protocol for TCF3 antibody 67140-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Reproduction

TCF3 regulates human endometrial stromal cell proliferation and migration in RPL.

Authors - Xiao-Wei Wei
  • KD Validated
View Article
humanWB,IHC

Mol Med Rep

Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis in vitro.

Authors - Nian Zhou
  • KD Validated
View Article
humanWB

Cancer Med

Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling

Authors - Xiong Shu
View Article
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