Validation Data Gallery
Product Information
The ChromoTek SUMO3-Trap Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-SUMO3 NANOBODY®/VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of SUMO3 and SUMO2 proteins from cell extracts of various organisms.
| Description | The SUMO3-Trap Magnetic Agarose Kit contains SUMO3-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of SUMO2 and SUMO3 proteins. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to human SUMO2 and SUMO3 (Small Ubiquitin-like Modifier 2 and 3). The ChromoTek SUMO3-Trap Agarose will immunoprecipitate both monovalent SUMO2/3 and chains formed by the process of SUMOylation. In addition to human SUMO2 und SUMO3, it will also bind to homologs from other mammals. |
| Binding Capacity | 25 ug of SUMO3 protein (~40 kDa) per 25 uL bead slurry |
| Conjugate | Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lammli), 200 mM glycine pH 2.5 |
| Wash Buffer Compatibility | 0.5 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 1 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | / |
| Compatibility with mass spectrometry | The SUMO3-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3716418 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze! |
Kit components
| Component | Description |
|---|---|
| SUMO3-Trap Magnetic Agarose | 20 reactions (500 µl) |
| Lysis buffer | Optimized for cytoplasmic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |