Validation Data Gallery
Product Information
The ChromoTek SUMO3-Trap Agarose consists of an anti-SUMO3 NANOBODY®/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of SUMO3 and SUMO2 proteins from cell extracts of various organisms.
| Description | Immunoprecipitation of endogenous SUMO2 and SUMO3 proteins with anti-SUMO3 Nanobody conjugated to agarose beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to human SUMO2 and SUMO3 (Small Ubiquitin-like Modifier 2 and 3). The ChromoTek SUMO3-Trap Agarose will immunoprecipitate both monovalent SUMO2/3 and chains formed by the process of SUMOylation. In addition to human SUMO2 und SUMO3, it will also bind to homologs from other mammals. |
| Binding Capacity | 25 ug of SUMO3 protein (~40 kDa) per 25 ul bead slurry |
| Conjugate | Agarose beads; ~90 um (cross-linked 4% agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5 |
| Wash Buffer Compatibility | 0.5 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 1 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | / |
| Compatibility with mass spectrometry | The SUMO3-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3716417 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze! |