• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated STK3 Monoclonal antibody

STK3 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse , Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C11A4

Cat no : CL488-66637

Synonyms

FLJ90748, KRS1, MST 2, MST2, STE20 like kinase MST2, STK3

Validation Data Gallery

Applications:
  • IF Staining of NIH/3T3 using CL488-66637

    IF Staining of NIH/3T3 using CL488-66637

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 STK3 antibody (CL488-66637, Clone: 1C11A4 ) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL488-66637

    FC experiment of NIH/3T3 using CL488-66637

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human STK3 (CL488-66637, Clone:1C11A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy STK3 Ready-To-Use IHC Kit. STK3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66637-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66637 targets STK3 in IF, FC (Intra) applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STK3 fusion protein Ag2741 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 3 (STE20 homolog, yeast)
Calculated molecular weight 491 aa, 56 kDa
Observed molecular weight 56-63 kDa
GenBank accession numberBC010640
Gene symbol STK3
Gene ID (NCBI) 6788
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 STK3 antibody CL488-66637Download protocol
FC protocol for CL Plus 488 STK3 antibody CL488-66637Download protocol
Standard Protocols
Click here to view our Standard Protocols