• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

STK3 Monoclonal antibody

STK3 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse , Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C11A4

Cat no : 66637-1-Ig

Synonyms

KRS1, MST 2, MST2, STE20 like kinase MST2, STK3

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 66637-1-Ig

    WB analysis of HeLa using 66637-1-Ig

    WB result of STK3 antibody (66637-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK3 transfected HeLa cells.

  • WB analysis using 66637-1-Ig

    WB analysis using 66637-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66637-1-Ig (STK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 66637-1-Ig

    IF Staining of NIH/3T3 using 66637-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using STK3 antibody (66637-1-Ig, Clone: 1C11A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IF detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy STK3 Ready-To-Use IHC Kit. STK3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66637-1-Ig targets STK3 in WB, IF, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STK3 fusion protein Ag2741 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 3 (STE20 homolog, yeast)
Calculated molecular weight 491 aa, 56 kDa
Observed molecular weight 56-63 kDa
GenBank accession numberBC010640
Gene symbol STK3
Gene ID (NCBI) 6788
RRIDAB_2881996
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STK3 antibody 66637-1-IgDownload protocol
IF protocol for STK3 antibody 66637-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols