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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite® Plus 488-conjugated ST6GALNAC6 Monoclona (CL488-66209)

    IF Staining of human colon cancer using CL488-66209

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 ST6GALNAC6 antibody (CL488-66209, Clone: 1A7B7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated ST6GALNAC6 Monoclona (CL488-66209)

    IF Staining of U2OS using CL488-66209

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 ST6GALNAC6 antibody (CL488-66209, Clone: 1A7B7 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated ST6GALNAC6 Monoclonal antibody

ST6GALNAC6 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-66209
Clone No.1A7B7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Synonyms

Sialyltransferase 7F, RP11 203J24.3, hST6GalNAc VI, GalNAc alpha-2,6-sialyltransferase VI, EC:2.4.99.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66209 targets ST6GALNAC6 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ST6GALNAC6 fusion protein Ag8358 相同性解析による交差性が予測される生物種
Full Name ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
Calculated molecular weight333aa,38 kDa; 374aa,41 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC007802
Gene Symbol ST6GALNAC6
Gene ID (NCBI) 30815
RRIDAB_2919299
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ969X2
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ST6GALNAC6 is a sialyltransferase that is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ST6GALNAC6 antibody CL488-66209Download protocol
Standard Protocols
Click here to view our Standard Protocols