Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | mouse colon tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66209-1-Ig targets ST6GALNAC6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8358 Product name: Recombinant human ST6GALNAC6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 67-333 aa of BC007802 Sequence: VFHYGSLRGRSRRPVNLKKWSITDGYVPILGNKTLPSRCHQCVIVSSSSHLLGTKLGPEIERAECTIRMNDAPTTGYSADVGNKTTYRVVAHSSVFRVLRRPQEFVNRTPETVFIFWGPPSKMQKPQGSLVRVIQRAGLVFPNMEAYAVSPGRMRQFDDLFRGETGKDREKSHSWLSTGWFTMVIAVELCDHVHVYGMVPPNYCSQRPRLQRMPYHYYEPKGPDECVTYIQNEHSRKGNHHRFITEKRVFSSWAQLYGITFSHPSWT 相同性解析による交差性が予測される生物種 |
| Full Name | ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 |
| Calculated molecular weight | 333aa,38 kDa; 374aa,41 kDa |
| Observed molecular weight | 38-42 kDa |
| GenBank accession number | BC007802 |
| Gene Symbol | ST6GALNAC6 |
| Gene ID (NCBI) | 30815 |
| RRID | AB_2881600 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q969X2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
| IHC protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
| WB protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
